Chamberlain played for the Oklahoma Sooners from 2012 - 2015 As a freshman in 2012, she set the Big 12 conference's single-season home run record, hitting thirty home runs; she finished second in the nation in home runs that year. She was three times-named Big 12 Player of the Week, becoming the first freshman in Big 12 softball history to win the award three times. As a true freshman, she led her team in home runs, RBI, runs scored, and total bases; second in walks; and third in batting average, hits, doubles, and stolen bases. Following the season, she was named to the all-conference first team and a first-team all-American.
In 2013, Chamberlain led the nation in four statistical categories, including home runs; home runs per game; slugging percentage; and runs per game. She ranked in the top ten nationally in four other statistical categories, including on-base percentage and batting average. She set eight single-season school offensive records, including for batting average; slugging percentage; on-base percentage; runs scored; RBI; extra-base hits; total bases; and walks. Chamberlain helped lead the Sooners to the National Championship in 2013. Following the season, she was again named a first-team all-American and a member of the all-conference first team.
In 2014, Chamberlain hit twelve home runs and led her team in on-base percentage and walks. Her fifty-one (51) walks is the sixth-best mark in Big 12 conference history. Despite time missed due to injury, she was named a third-team all-American after the season.
Entering 2015, Chamberlain needed eighteen home runs to tie the all-time record. She led the team in six statistical categories, including runs, RBI, slugging percentage, and on-base percentage; she led the Big 12 conference in on-base percentage; slugging percentage; walks; and runs per game. On April 30, 2015, Chamberlain hit a grand slam in a game against North Texas pitcher Kenzie Grimes; with the home run, the 91st of Chamberlain's career, she moved into sole possession of first place all-time on the NCAA career home runs list. Following her senior season, Chamberlain was nominated for the Honda Cup Award and was named the Big 12 conference's Female Athlete of the Year. She was named to both the all-American first-team as well as the Big 12 first-team.
Chamberlain played for Team USA in 2013. She previously spent two years (2010-11) as a member of the Junior Women's National Team and was named the 2010 USA Softball Athlete of the Year for the JWNT.
Chamberlain was selected with the #1 overall draft pick in the 2015 NPF Draft by the USSSA Pride. In thirty-eight games with the Pride during the 2015 summer season, Chamberlain hit .205 with five home runs and eleven RBI.